ISSN 1995-5537

"Biotechnology" journal V. 5, No. 2, 2012
Р. 76-85, Bibliography 17, Ukrainian.
Universal Decimal classification: 615.361.018

PHENOTIPIC CHARACTERISTIC OF CELL CULTURE DERIVED
FROM CRYOPRESERVED CHORION TISSUE

V. A. Shablii, M. D. Kuchma, V. M. Kyryk, L. L. Lukash, G. S. Lobintseva

Institute of molecular biology and genetics of National Academy of Science of Ukraine, LLC Institute of Cell Therapy,
State institute of genetics and regenerative medicine Academy of Medicine of Ukraine

It was shown possibility of cell culture obtaining from cryopreserved chorion tissue using 1.5 M DMSO. For the first time we described features of the cell culture formation from cryopreserved human chorion and its immunophenotype. We described the character of CD90 marker expression in cultures of the cells derived from cryopreserved chorion tissue. Decreasing of expression of the markers CD90 and CD105 was observed together with drop in proliferative activity and acquisition of multinuclearity for the cells. The population of endothelial progenitor cells in cell culture was detected on the first passage. Expression of cytokeratins was found in populations of the cells derived from native and cryopreserved chorion tissue.

Key words: chorion, cryopreservation, cell culture, multipotent mesenchymal stromal cells.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008