Obtaining and characterization of isolated root culture from plants of genus Gentiana Konvalyuk I. I., Hrytsak L. R., Mel’nyk V. M., Drobyk N. M., Kunakh V. A.

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ISSN 1995-5537

"Biotechnology" journal V. 4, No. 3, 2011
Р. 29-35, Bibliography 22, Ukrainian.
Universal Decimal classification: 576.5: 582.923.1

Obtaining and characterization of isolated root culture
from plants of genus Gentiana

Konvalyuk I. I., Hrytsak L. R., Mel’nyk V. M., Drobyk N. M., Kunakh V. A.

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
Volodymyr Hnatiuk Ternopil’ National Pedagogical University

Conditions for generation and long-term maintenance of isolated root cultures from plants Gentiana acaulis, G. asclepiadea, G. cruciata, G. lutea, G. рneumonanthe and G. punctata have been specified. Cultures were substantially differed from each other by the growth parameters: number and length of lateral roots, growth index and biomass yield. Highly intensive growth and considerable biomass yield was achieved via two-step maintenance in hormonal and then in phytohormone free medium in most cultures. Among the specimens examined the highest biomass yield showed plants G. lutea — 225 g per 1 L of nutrient medium in 28–42 days of growth that corresponded to a root mass of 10–12 years old plant in nature. Relationship of ability to form an isolated root culture and the original genotype, phytohormone and mineral composition of the nutrient medium was revealed.

Key words: Gentiana L., isolated root culture, growth index, biomass yield.