Select your language

ISSN 1995-5537

"Biotechnology" journal V. 4, No. 3, 2011
Р. 36-42, Bibliography 30, Ukrainian.
Universal Decimal classification: 543.553+543.632.518+577.152.4

Development of amperometric microbiosensor for D-serin determination

Soldatkin O. O.

Institute of Molecular Biology and Genetics of National Academy of Science of Ukraine, Kyiv

A laboratory prototype of the microbiosensor for determination of D-serine concentration has been developed. The bioselective membrane on the basis of D-aminoacids oxidase, immobilized with BSA on the surface of amperometric microtransducer, has been used as a sensitive element of the biosensor. Dependence of the biosensor response on buffer concentration and temperature has been studied while optimizing the biosensor function. The microbiosensor developed has been shown to be stable, its response — reproducible, linear range of the substrate determination — within D-serine concentrations of 0–0.8 mМ, minimum measurable limit — 2 мМ. The amperometric microbiosensor developed can be used as well for selective monitoring for further experiments to optimize and adapt his work to determine the concentration of D-serine level in different biological liquids both in vitro and in vivo.

Key words: D-serine, D-aminoacid oxidase, amperometric transducer, microbiosensor, in vitro and in vivo analysis.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008