ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ

V. 3, No. 6, 2010

"Biotechnology" journal Vol. 3, No. 6, 2010
Р. 49-58, Bibliography 23, Ukranian.
Universal Decimal classification: 615.339:582.288:576.858

FODDER AND BAKER YEASTS USING FOR PRODUCTION
OF BIOLOGICALLY ACTIVE GLYCANS

V. S. Podgorsky, O. G. Kovalenko, V. N. Vasyliev, V. O. Isakova

Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv

Fodder (Candida maltosa) and baker (Saccharomyces cerevisiae) yeasts using for biologically active glycans obtaining have been studied. The isolated glycans contained homopolysaccharides mannans and glycans. The enzyme- (yield 14%) and autolysates (yield 8,5%) appeared to be perspective for obtaining the biologically active polysaccharides. The intact cells of baker yeasts were less suitable (yield of extracted glucan using the proposed technology was 7,2%). The isolated glycans contained 75–94% of active element and showed the marked inhibiting effect against the tobacco mosaic virus infectiveness and Agrobacterium tumefaciens induction of tumours in the explants of potatoes tubers parenchime. The glycan preparations stimulated germinating power, energy of seeds sprouting, growth and development of tomatoes and tobacco. They may be used in elaboration of complex biopreparations intended for control of virus and bacterial diseases of agricultural plants and in medicine.

Key words: yeasts, Candida maltosa, Saccharomyces cerevisiae, glycans, biological activity, tobacco mosaic virus, Agrobacterium tumefaciens, tobacco, tomatoes, potato.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008