ISSN 1995-5537
"Biotechnology" journal Vol. 3, No. 5, 2010
Р. 83-91, Bibliography 21, Ukrainian.
Universal Decimal classification: 582.284:547.458:54.053:632.953
О. G. Kovalenko, E. N. Polishchuk, S. P. Wasser
Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv
Kholodny Institute of Botany of National Academy of Sciences of Ukraine, Kyiv
High fungi ability to produce glycans which are active inhibitors of plant virus infection has been shown in three strains of G. adspersum isolated in Georgia, China and Israel. The antiviral preparations may be obtained using water, acid or alkaline extraction. Ability of G. adspersum glycan to activate the plants protective mechanisms and to inhibit the infectious and reproductive functions of tobacco mosaic virus (TMV) has been determined. Resistance of tobacco to TMV infection under glycan influence is formed de novo by participation of the mechanism of RNA transcription on the cellular DNA matrix which are sensitive to influence of specific inhibitor actinomycin D. At the same time, ability of polysaccharide to suppress reproduction of TMV in plants with systemic viral infection has been found.
Key words: Basidiomycetes, Ganoderma adspersum, glycans, antiviral activity, tobacco mosaic virus, virus resistance of plants, actinomycin D, Nicotiana tabacum, Datura stramonium.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008