ISSN 1995-5537
"Biotechnology" journal Vol. 3, No. 4, 2010
Р. 67-74, Bibliography 29, Russian.
Universal Decimal classification: 579.254.2
А. G. Komisarenko, S. I. Mykhalska, A. V. Kochetov, O. M. Tishchenko
Institute of Plant Physiology and Genetics of National Academy of Science of Ukraine, Kyiv;
Institute of Cytology and Genetics, Russian Academy of Science, Novosibirsk
The induction of regeneration in vitro of sunflower inbred lines (Helianthus annuus L.) under Agrobacterium-mediated transformation by strain LBA 4404 harboring antisense suppressor for proline dehydrogenase with gene neomycinphosphotransferase II were investigated. After cocultivation, significant decreasing of forthputting frequency (FF) under direct organogenesis out of a segment of seedling (half cotyledon, hypocotyl 1–2 mm size) 3–4-days after germinating was shown. It was found out that regeneration frequency depended on genotype as well as both selective, bactericide agents and ultrasound. On the whole, kanamycin (Kan, 100 mg/l) has shown negative effect, cefotaxime-positive effect (Cf, 500 mg/l) on FF while sonication (15 s, 44 kHz) increased significantly the realization of morphogenetic potential. It was proposed that procedure Kan- selection had to be done after lag-period of cultivation with bactericide agent.
Key words: Helianthus annuus L., regeneration in vitro, sonication, thiosulfate Na, Agrobacterium tumefaciens.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008