CONCERNING THE PECULIARITIES OF GENE EXPRESSION CHANGES IN PLANT LEAF CELLS DURING TWENTY-FOUR-HOUR PERIOD V. A. Tsygankova

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ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 4, 2010
Р. 86-95, Bibliography 45, English.
Universal Decimal classification: 631.811.98 75.117.2.577.2.08

CONCERNING THE PECULIARITIES OF GENE EXPRESSION CHANGES IN PLANT LEAF CELLS DURING TWENTY-FOUR-HOUR PERIOD

V. A. Tsygankova

Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine, Kyiv

Using DOT-blot hybridization DNA with RNA, the analysis of degree homology of products of gene expression (cytoplasmic mRNAs) was fulfilled from the cells of dicotyledonous (soya, pea) and monocotyledonous plants (wheat, corn) leaves followed by their separation from plants in an early period with weak photosynthesis (at 5 a. m.), in a middle period (at 2 p. m.) with strong photosynthesis and in the evening period (at 9 p. m.) with weak photosynthesis towards mRNA from plant leaves being in a dark phase (at 2 a. m. — control). It was shown that divergence in mRNA populations between experience and control begins early in the morning at weak sunlight, increases sharply at daylighting (at 2 p. m.) and drew in at the sunset. Emistim C increases difference between control and 5-hour time-of-day to a level of 2-hour period. It is assumed that these changes occur by means of «windmill switching» the genes belonging to one family but having different activity due to their location under promoters of different force (promoters of weak, moderate and strong force) and such type of plasticity (maneuverability) of plant adaptation to the environment is achieved by such mechanism, and emistim C «switches on» genes under strong force promoters in the morning with expression up to the level shown under bright (strong) sunlight.

Key words: plant growth regulator emistim C, mRNA populations, photosynthetical activity.