ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 3, 2010
Р. 72-77, Bibliography 16, Russian.
Universal Decimal classification: 576.371:611.018.46

THE STUDY OF THE CAPABILITY TO DIFFERENTIATION IN OSTEOGENIC AND ADIPOGENIC DIRECTIONS OF HUMAN BONE MARROW MESENCHYMAL STROMAL CELLS OF CLONAL ORIGIN

N. G. Skorobogatova, Yu. A. Petrenko, N. A. Volkova, O. Yu. Petrenko

Institute for Problems of Cryobiology and Cryomedicine
of National Academy of Sciences of Ukraine, Kharkiv

Immunophenotype and differentiation potential of mesenchymal stromal cells (MSCs) derived from bone marrow (BM) was studied. Isolation of individual colonies allowed to investigate colony-forming and differentiation capacities of the cells within single subpopulations. The cells derived from large colonies showed high ability to expansion and induced mesenchymal differentiation into osteogenic and adipogenic directions. During cultivation in a mixture of osteogenic and adipogenic inductive media BM MSCs demonstrated a predominance of osteogenic differentiation.

Key words: bone marrow, mesenchymal stromal cells, differentiation.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008