ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 3, 2010
Р. 91-96, Bibliography 18, Ukrainian.
Universal Decimal classification: 577.113

MODIFICATION OF GENES OF STAT TRANSCRIPTION FACTORS
USING  BAC-RECOMBINATION METHOD

А. О. Tsyrulnyk, V. V. Snitynsky, R. S. Stoika

Lviv National Agricultural University;
Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv

The modified form of gene of transcription factor STAT3 which is a potent mediator of JAK/STAT signaling pathway was synthesized. For high level of expression of created transgene in mouse genome, a modern and effective method of BAC (Bacterial Artificial Chromosome) recombination was used. The method is based on recombination system of Rаc-prophage (Rec/ET) or l-phage (Red?/Red?) transferred into Escherichia coli DH10B cells. In case of BAC recombination, a DNA of big size (up to 350 kbp) can be modified and all the known regulatory regions essential for desired transgenic expression can be included and introduced into genome. This method is very convenient for modification of any genes of transcription factors and generation of transgenic animal models.

Key words: synthesis of DNA, STAT transcription factors, gene modification.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008