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ISSN 1995-5537

3 2010

"Biotechnology" journal Vol. 3, No. 3, 2010
Р. 58-65, Bibliography 9, Russian
Universal Decimal classification: 577.115:663.12

BIOSYNTHESIS OF LIPIDS BY RHODOTORULA GRACILIS YEASTS

S. M. Shulga, A. F. Tkachenko, N. E. Beyko, A. I. Khomenko, A. S. Andriyash

State organization «Institute of Food Biotechnology and Genomics»
of National Academy of Sciences of Ukraine, Kyiv

As a result of screening of different types of yeast cultures which are lipids producers the culure of Rhodotorula gracilis SK=4 was selected with a most fatty coefficient which is equal 8.0. The best growth and lipidformed activity of this producer was attained on the complex composition medium, containing molasses as basic substrate. It was revealed that by a factor, limiting growth and synthesis of R. gracilis SK=4 lipids, there was oxygen in medium. The lipids syntheis and their fatty acids composition could be regulated, changing a temperature, pH medium, and aeration intensity. The selection of medium and terms of cultivation gave a possibility to promote a fatty coefficient to 17.5–18.1.

Key words: yeasts, Rhodotorula gracilis, microbial lipids.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008