ASSESSMENT OF THE IN VIVO DNA-DAMAGING ACTION OF THE GOLD NANOPARTICLES OF DIFFERENT SIZES S. M. Dybkova, L. S. Rieznichenko, T. G. Gruzina, Z. R. Ulberg

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ISSN 1995-5537

3 2010

"Biotechnology" journal Vol. 3, No. 3, 2010
Р. 66-71, Bibliography 9, Russian.
Universal Decimal classification: 577.151:579.864.1

ASSESSMENT OF THE IN VIVO DNA-DAMAGING ACTION
OF THE GOLD NANOPARTICLES OF DIFFERENT SIZES

S. M. Dybkova, L. S. Rieznichenko, T. G. Gruzina, Z. R. Ulberg

F. D. Ovcharenko Institute of Biocolloidal Chemistry
of National Academie of Sciences of Ukraine, Kyiv

It is shown that gold nanoparticles which average size is 20 and 30 nm (in contrast to 10 and 45 nm nanoparticles) do not interact with blood serum albumin and are resistant to coagulation in model systems. By the method of alkaline gel-electrophoresis of single eukaryotic cells («comet-assay») it was shown that under single intravenous injection the gold nanoparticles of 20, 30 and 45 nm in size didn’t show DNA-damaging action in the cells of liver, kidneys, intestines and bone marrow of rats. 20 nm gold nanoparticles in contrast to 30 and 45 nm nanoparticles showed DNA-damaging action in spleen. These data suggest that 30 nm gold nanoparticles are the most biocompatible and biosafety.

Key words: gold nanoparticles, blood models system, serum albumin, coagulation, DNA-damage action, method of alkaline gel electrophoresis of single cells, biocompatibility, biosafety.