ISSN 1995-5537
"Biotechnology" journal Vol. 3, No. 1, 2010
Р. 35-40, Bibliography 14, Ukranian.
Universal Decimal classification: 577.151.083:615.917
National Taras Shevchenko University, Kyiv
Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv
Fibrino(geno)lytic enzyme «Blomulyse» from Agkistrodon blomhoffii ussuriensis venom has been isolated in three chromatography steps. The purified enzyme is a typical single-chain metalloproteinase with molecular weight of approximately 30 kDa. Obtained enzyme hydrolyzed with high-efficiency the Aα-chain of fibrinogen followed by the Bβ-chain and showed no effect on the γ-chain.
Using different model systems in vitro we indicated that fibrolyse has no effect on the platelets aggregation stimulated by collagen, but on the other hand it inhibited ADP- and adrenalin-induced platelet aggregation.
Key words: fibrino(geno)lytic enzyme, snake venom, Agkistrodon, fibrinogen, platelets.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008