RECOMBINANT ANTICOAGULANT FORM OF A HUMAN ?-THROMBIN M. V. Kolodzejskaja, V. I. Grishchuk, T. M. Chernyshenko, E. I. Jusova, T. V. Grinenko

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 3, 2009
Р. 9-22, Bibliography 102, Russian.
Universal Decimal classification 577.157.2

RECOMBINANT ANTICOAGULANT FORM OF A HUMAN α-THROMBIN

M. V. Kolodzejskaja, V. I. Grishchuk, T. M. Chernyshenko, E. I. Jusova, T. V. Grinenko

Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine

Mutant thrombin forms that cause essential changes of an active center geometry, blocking of the subsites of the substrata linkage located in separate loops of a molecule of enzyme that results in change of conformational mobility of a molecule and hence an increased activity of enzyme are discussed in the review. Question concerning thrombin allostery is important for identification of interrelation between its structure and thrombin functions and also for development of a new direction in therapy.

Key words: recombinant thrombin, allostery, mutagenesis, protein C, thrombomodulin, anti coagulant function.