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ISSN 1995-5537

2 2009

"Biotechnology" journal Vol. 2, No. 2, 2009
Р. 78-85, Bibliography 13, Ukrainian.
Universal Decimal classification: 577(087.1+112.4)

Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv
 

Approach for deriving conjugates of gold nanoparticles with Staphylococcus aureus protein A recombinant fragment for immunochromatograpical test-systems was optimized. Influence of main components in reaction solution on particle size of gold and its conjugates with protein A fragment, their homogeneity, stability and immunoglobulin-recognition ability was investigated.

Methods for rapid size determination of gold nanoparticles based on their optical characteristics were proposed. Recommendations for deriving staphylococcal protein A fragment-gold nanoparticle conjugates that could be successfully used in fast immunochromatograpical test-systems were given.

Key words: gold nanoparticles, protein А, Staphylococcus aureus, immunoglobulin recognition ability, immunochromatograpical test-systems, electron microscopy, laser correlation spectroscopy, spectrophotometry, RGB spectrum.
 

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008