CLONNING AND EXPRESSION OF FUNCTIONAL FRAGMENT D-E-A-A* OF PROTEIN А FROM Staphylococcus aureus O. S. Oliynyk, D. V. Kolibo, А. А. Kaberniuk, A. Yu. Labyntsev N. V. Korotkevich, S. I. Romanyuk, S. V. Komisarenko

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 59-68, Bibliography 35, Ukraininan.
Universal Decimal classification: 577(с87.1+112.4)

CLONNING AND EXPRESSION OF FUNCTIONAL FRAGMENT D-E-A-A*
OF PROTEIN А FROM Staphylococcus aureus

O. S. Oliynyk, D. V. Kolibo, А. А. Kaberniuk, A. Yu. Labyntsev
N. V. Korotkevich, S. I. Romanyuk, S. V. Komisarenko

Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine

Recombinant fragment of protein A from Staphylococcus aureus with unique sequence, which has four domains D-E-A-A* and is expressed in Escherichia coli have been obtained. Conjugates of recombinant fragment with horse radish peroxidase and colloidal gold nanoparticles were used for testing its functional activity and immunoglobulin-binding properties.

It was shown, that recombinant fragment of protein A marked by horseradish peroxidase or colloidal gold nanoparticles, has the same specificity to mammal immunoglobulines as native protein A. Therefore an obtained analog of protein A can be used not only in development of chromatography sorbents for immunoglobulins purification and immunochemical conjugates for immunoglobulins detection, but also in fundamental investigations of immunobiological properties of protein A.

Key words: protein A, Staphylococcus aureus, recombinant analog, immunoglobulin-binding activity, immunoenzyme assay, immunochromatography tests.