ISSN 1995-5537
"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 117-123, Bibliography 11, Ukrainian.
Universal Decimal classification 541.18.045+577.15
α-AMILASE IMMOBILIZATION ON CELLULOSE MEMBRANES
MODIFIED BY CHITOSANE AND СIBACRON BLUE F3G-A
National University of Kiev Mohyla-Academy, Membrane Research Center, Kуiv
Ukrainian research institute of alcohol and biotechnology of foods, Kyiv
A method of the affinity membranes producing based on chitosane covalent bonding with cellulose membranes surface and Cibacron Blue F3G-A dye fixing as affine ligand has been developed. Transport and surface properties of the modified membranes have been studied. Optimum α-amilase immobilization is found to be the following: pH and solution concentration are 3.5 and 1 mg/sm3 respectively. Starch conversion of unregenerated membranes with immobilized enzyme was up to 84%.
Key words: enzyme immobilization, α-amylase, affine membranes.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008