Select your language

ISSN 1995-5537

4 2008

"Biotechnology" journal Vol. 1, No. 4, 2008
Р. 76-84, Bibliography 20, Ukrainian.
Universal Decimal classification 577.15.543.555

Institute of Molecular Biology and Genetics of  the National Academy of Sciences of Ukraine, Kyiv
National Taras Shevchenko University of Kyiv
National Food Industry University, Kyiv
Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine, Kyiv

Enzyme conductometric biosensor for lactose determination has been developed. Three-enzyme membrane (glucose oxidase, mutarotase, β-galactosidase) immobilized on the surface of conductometric transducer was used as biosensitive element. The analytical characteristic of the biosensor obtained were investigated. The time of lactose analysis in solution was 1–2 minutes, linear range was from 0,01 mM to 0,75 mM for glucose determination and from 0,01 mM to 1,25 mM for lactose determination. Dependence of responses of biosensor to substrate on pH, ionic strength, buffer capacity of work solution were studied, and the data of selectivity and storage stability were presented.

The developed conductometric biosensor is featured in high operational stability and signal reproducibility.

Key words: conductometric enzyme biosensor, lactose, glucose, glucose oxidase, mutarotase, β-galactosidase.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008