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ISSN 1995-5537

3  2008

“Biotechnology” journal Vol. 1, No. 3, 2008
P. 56-63, Bibliography. 13, укр.
Universal Decimal classification: 581.143.6:576.5+663.1

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv

E. plantagineum tissue culture has been generated from the roots of seed shoots on 5C01 agarized nutrient medium. Cultivation on the LS-m nutrient medium induced biosynthesis of the red shikonin pigments. Variant of callus tissue (3Ep), accumulating 2.11% of shikonin derivatives per dry biomass whose yield on the 14th day of growth constituted up to 16 mg/L of nutrient medium was selected. Through the thin layer chromatography (in Sorbfill UV 254 plates) and column chromatography (Silica gel 60) there was evaluated the spectrum of shikonin derivatives in the course of culture establishment. Organogenic culture at the first steps of culturing on the LS-m nutrient medium was found to synthesize alongside with red fractions of shikonin pigmentes the blue ones as well. But after 1,5 year of cultivation Echium callus cells synthesized exclusively red fractions of shikonin pigments.

Key words: Echium plantagineum L., plant tissue culture, red pigments, shikonin derivatives, cell lines, producents of biologically active substances.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008