ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta Т. 18, No. 2, 2025
P.  , Bibliography 9 , Engl.
UDC 602.4+604+663.1

EFFECT OF B VITAMIN ADDITION ON THE BIOSYNTHESIS OF LACTIC ACID BY LACTOBACILLUS DELBRUECKII

Kiiv D.I., Vasylyuk S.V.

Lviv Polytechnic National University Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology

Aim. The main objective of this work is to investigate the effect of B vitamins on the biosynthesis of lactic acid (LA) by the Lactobacillus delbrueckii UY-2/13 strain when using corn steep liquor as the sole nitrogen source.

Methods. The influence of B vitamins was assessed using a one factor experimental design. The lactic acid concentration in the culture broth at the end of fermentation was determined using high-performance liquid chromatography while reducing sugars were analyzed by the modified Bertrand method.

Results. The study revealed that riboflavin (B2) at a concentration of 1 mg/L increased lactic acid production by approximately 6%, whereas calcium pantothenate (B5) reduced it by about 4%. Other vitamins (PP, B9, B1, B6, H) had no significant effect on LA biosynthesis.

Conclusions. Vitamins B2 and B5 have a certain effect on the biosynthesis of lactic acid by Lb. delbrueckii UY-2/13. Other B vitamins (PP, B9, B1, B6 and H) do not significantly influence the biosynthesis of lactic acid.

Keywords: Lactobacillus delbrueckii, lactic acid biosynthesis, В vitamins, cofactor supplementation, vitamin influence.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2025