ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 18, No. 2, 2025
P. 38-40, Bibliography 9 , Engl.
UDC 577.218+616-006
ADAPTOR PROTEIN CIN85 POTENTIATES THE MOTILITY OF OSTEOSARCOMA CELLS
I.Horak1, 2, T.Skaterna 2, M.PřikrylL1, J. Navrátilová1, 3, J. Šmarda1, L. Drobot2, P. Beneš1, 3, L. Knopfová1, 3
1 Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic;
2 Department of Cell Signaling, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine;
3 International Clinical Research Center, Brno, Czech Republic.
Osteosarcoma (OSA) is the most common primary malignant bone tumor in children and adolescents, characterized by high metastatic potential and poor prognosis. The adaptor protein CIN85 is known to be upregulated in various cancers and is involved in cell motility and invasion.
Aim. This study was purposed to investigate the role of CIN85 in the migra-tion of osteosarcoma cells.
Methods. In vitro, scratch assay, xCELLigence, and Transwell assay were used to evaluate cell migration, while RNA-seq, qPCR, and Western blotting assessed gene expression.
Results. Public datasets revealed elevated CIN85/SH3KBP1 expression in OSA tissues compared to normal bone, with even higher levels observed in metastases. Functional studies using CIN85-overexpressing and CIN85-silenced HOS and SAOS-2 osteosarcoma cells demonstrated that CIN85 promotes OSA cell migration and invasion in both 2D and 3D models. RNA-seq analysis identified differentially expressed genes and enriched pathways related to migration, extracellular matrix, adhesion, and cell signaling. CIN85-driven motility was shown to depend on the expression of COL3A1 and MMP2, Akt/mTOR signaling, and NOX activity.
Conclusion. These findings support CIN85 as a potential biomarker and therapeutic target in osteosarcoma.
Keywords. Osteosarcoma, migration, metastasis, CIN85.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2025