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SSN 2410-7751 (Print)
ISSN 2410-776X (Online)


iotechnologia Acta  Т. 15, No. 1, 2022
P. 72-80. Bibliography 22, Engl.
UDC: 579.6:604


 T. S. Todosiichuk 1, O. V. Pokas 2, S. О. Soloviov 3, T. S. Ryzhkova 1

1Igor Sikorsky Kyiv Polytechnic Institute, Kyiv
2SI “L.V. Gromashevsky Institute of Epidemiology and Infectious Diseases of NAMS of Ukraine”, Kyiv
3 Shupyk National Healthcare University of Ukraine, Kyiv

Aim. Evaluation of biotechnological aspects of the production of a liquid formulation of the multifunctional antiseptic preparation of microbial origin, which can be typified as an enzybiotic; characterization of the enzyme specificity of the studied formulation, stabilization methods, its ability to affect microbial biofilms.

Methods. Gel-filtration and electrophoresis were used to study the component composition and the specificity of the enzyme complex of the Streptomyces albus UN 44 producer strain. Proteolytic and staphylolytic activities of individual fractions were determined. The Pseudomonas aeruginosa biofilm and its formation under the effect of various drug formulations were quantitatively evaluated by spectrophotometry.

Results. The stability of the liquid formulation of the enzybiotic biosynthesized by S. albus UN 44 was demonstrated. Activity of the formulation could be prolonged and additionally stabilized by adding of 0.5% polyvinyl alcohol. Fractionation of the formulation enzyme complex using gel-filtration revealed the presence of at least three proteinases of different molecular weights (80-100, 24-35 and 20 kDa) and lysoenzymes (18-22 kDa). The effectiveness of the developed liquid antibiotic formulation for the destruction and inhibition (8-10 folds) of the biofilm formation by clinical strains of P. aeruginosa was shown.

Conclusions. The broad spectrum, multidirectional mechanisms of antimicrobial and regenerative action of enzybiotic drug, and the possibility of its production directly from the biotechnological process determine the prospects of its manufacturing and use as a multifunctional surface antiseptic.

Key words: antibiotic, antimicrobial action, Streptomyces albus, liquid formulation, biofilms, pathogens.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2022