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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

5 2021 200ps

Biotechnologia Acta  Т. 14, No. 5 , 2021
P. 56-62, Bibliography 31, Engl.
UDC: 577.118


 Palonko R.I., Pavlyuk O.V., Arnauta O.V., Kalachniuk L.H.

National University of Life and Environmental Sciences of Ukraine

Aim. The Department of Biochemistry and Physiology of Animals, named after Academician Guly NUBIP of Ukraine, developed magnesium and phosphorus combined medication based on casein. Our aim was to test its bioavailability based on the ability to be hydrolyzed by a mixture of pancreatic digestive enzymes trypsin and chymotrypsin, also check the absence of cytotoxic effects on cell cultures.

Methods. To assess bioavailability, we used hydrolysis of the medication with a mixture of trypsin and chymotrypsin, followed by detection of hydrolysis products by polyacrylamide gel electrophoresis. A standard MTT-test performed on both MT-4 and Namalva cell lines was used to assess cytotoxic effects.

Results. Based on electrophoresis data, it was found that despite chemical modifications of the natural casein, the medication based on it is characterized by a high ability to hydrolyze by digestive enzymes under the same conditions as casein. Also, an MTT-test demonstrates that the medication has no cytotoxic properties against cell lines MT-4 and Namalva.

Conclusions. Since the negative effects of the drug associated with its digestibility and toxicity have not been observed, it is recommended to continue the study of its effects on living organisms.

Key words: magnesium, phosphorus, casein, chelate, in vitro, hydrolysis, cell culture, cytotoxicity, MTT reagent, NADH (nicotinamide adenine dinucleotide).

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2021