ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 11, No 5, 2018
https://doi.org/10.15407/biotech11.05.054
54-64Р. , Bibliography 63, English
Universal Decimal Classification: 579:662.7
THE STRUCTURE AND PROPERTIES OF MICROBIOCENOSIS IN DUMPS OF THE FUEL AND ENERGY COMPLEX OF UKRAINE
I. A. Blayda, T. V. Vasylieva, L. I. Sliusarenko, S. N. Shuliakova, V. F. Кhitrich
Odesa National Mechnykov University, Ukraine
The work aimed to conduct complex chemical and microbiological study of the dumps of the fuel and energy complex of Ukraine. It is established that the qualitative composition of the aboriginal microbiota of the studied technogenic substrates does not to depended on the storage time, because it was determined by the chemical and mineralogical compositions and is mainly represented by the heterotrophic and acidophilic chemolithotrophic bacteria (AСB). It is noted that the number of all groups of microorganisms in dumps increased during long term storage due to internal processes and the impact of external climatic factors. In our experiment the ACB association demonstrated the maximum leaching activity when the divalent iron was as an energy source. It is also noted that the “silicate” bacteria present in the aboriginal consortium and have no leaching activity, significantly increase bioleaching rates by ACB. The results of the study indicate on the formation of resistant specific microbiocenoses in the dumps of the fuel and energy complex that can be used as sources of highly active strains obtaining for use in biotechnological processes of metal extraction.
Key words: aboriginal community, dumps, bioleaching
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
References
1. Galetskii L. S., Naumenko U. Z., Pilipchik A. D. Technogenic deposits are a new non-traditional source of mineral raw materials in Ukraine. Ekolohiia dovkillia ta zabezpechennia zhyttediialnosti. 2002, 5(6), 77–81. (In Ukrainian).
2. Pashkov G. L., Saykova S. V., Kuz’min V. I. Ash of natural coals is an unconventional source of raw materials of rare elements. Zhurnal Sibirskogo federal’nogo universiteta. Seriia: Tekhnika i tekhnologii. 2012, V. 6, P. 520–530. (In Russian).
3. Blayda І. A. Extraction of valuable metals during industrial wastes processing by biotechnological methods (Review). Energotehnologii i resursosberezhenie. 2010, V. 6, P. 39–45. (In Russian).
4. Blaida I. A., Vasileva T. V., Sliusarenko L. I., Khitrich V. F., Ivanytsia V. A. Extraction of rare and nonferrous metals by microbial communities of the ash from burning Pavlograd’s coal. Mikrobiologiya i Biotekhnologiya. 2012,V. 3, P. 91–101. (In Russian).
5. Karavayko G. I., Kuznetsov S. I., Golomzik A. I. The role of microorganisms in leaching metals from ores. Moskva: Nauka. 1972, 248 p. (In Russian).
6. Ivanov M. V., Karavayko G.I . Geological microbiology. Mikrobiologiya. 2004, 73(5), P. 581–597. (In Russian).
7. Tolstov E. A., Latyshev V. E., Lil’bok L. A. Possibilities of using biogeotechnology in leaching of poor and refractory ore. Gornyj zhurnal. 2003, V. 8, P. 63–65. (In Russian).
8. Methods for General Bacteriology. V. 2. Moskva: Mir. 1984, 265 p. (In Russian).
9. Karavayko G. I., Rossi Dzh., Agate A. Biotechnology of metals. A Practical Guide. Moskva: Tsentr mezhdunarodnykh proektov GKNT. 1989, 375 p. (In Russian).
10. Khavezov I., Tsalev D. Atomic Absorption Analysis. Leningrad: Khimiya. 1983, 144 p. (In Russian).
11. Vasileva T. V., Blaida I. A., Ivanytsia V. A. The main groups of microorganisms involved in the biohydrometallurgical process. Problemy ekolohichnoi biotechnologii. Available at http://jrnl.nau.edu.ua/index.php/ ecobiotech/article/view/4678 (accessed, June, 2013)
12. Shuang Mi, Jian Song, Jianqun Lin, Yuanyuan Che, Huajun Zheng, Jianqiang Lin. Complete Genome of Leptospirillum ferriphilum ML-04 Provides Insight into Its Physiology and Environmental Adaptation. The Microbiological Society of Korea. 2011, 49(6), 890–901. https://dpi.org/10.1007/s12275-011-1099-9
13. Giaveno A., Lavalle L., Chiacchiarini P., Donati E. Bioleaching of zinc from lowgrade complex sulfide ores in an airlift by isolated Leptospirillum ferrooxidans. Hydrometallurgy. 2007, 89(1–2), 117–126. https://dpi.org/10.1016/j.hydromet.2007.07.002
14. Andreyuk E.I., Kozlova I.A. Lithotrophic bacteria and microbiological corrosion. Kiyv: Naukova dumka. 1977, 164 p. (In Russian).
15. Zhou Qiu Guan, Bo Fu, Hong Bo Zhou. Isolation of a strain of Acidithiobacillus caldus and its role in bioleaching of chalcopyrite. World J. Microbiol. Biotechnol. 2007, 23(9), 1217–1225.
16. Kukanova S. I. Heterotrophic microorganisms and their role 3 processes of gold extraction from non-standard raw materials. Ph.D. dissertation. Dept. Rudnoi mikrobiologii i bio-geotehnologii. Institut mikrobiologii Respubliki Uzbekistan, Tashkent, 1992. (In Russian).
17. Karavayko G. I., Belkanova N. P., Eroshchev-Shak V. A., Avakyan Z. A. The role of microorganisms and some physicochemical factors of the environment in the destruction of quartz. Mikrobiologiya. 1984. V. 53 (6), P. 976–981. (In Russian).
18. Torma A. E. The role of Thiobacillus ferrooxidans in hydrometallurgical processes. Adv. Biochem. Engin. 1977, 6, 1–37. https://dpi.org/10.1007/3-540-08363-4_1
19. Tributsch H. Direct vs indirect bioleaching. Hydrometallurgy. 2001, 59(2-3), 177–185. https://dpi.org/10.1016/S0304-386X(00)00181-X
20. Sokolova G. A., Karavayko G.I. Physiological and geological activity of thiobacteria. Moskva: Nauka. 1964, 332 p. (In Russian).
21. Blaida I. A., Vasileva T. V., Semenov K. І., Baranov V. I., Ivanytsia V. A. A two-stage method of bioleaching of gallium and germanium. UA Patent 104268. January 25, 2016. (In Ukrainian).