"Biotechnologia Acta" V. 10, No 1, 2017
https://doi.org/10.15407/biotech10.01.043
Р. 41-51, Bibliography 16, English
Universal Decimal Classification: 612.397:661.725.4
Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
The aim of the study was to evaluate changes in the portion of polar and neutral lipids in the cells of Clostridium during their cultivation in the presence of butanol. Four natural isolates of Clostridium genus were studied with flow cytometry approaches. Under the optimal culture conditions, the polar lipids prevailed over neutral ones in bacterial cells; the content of neutral lipids doubled in spores of these microorganisms, while the content of polar ones was reduced. Strains No 1 and No 2 were able to grow at 1% butanol in the medium, and the strain No 4 was at 1.5%. When cultivated in the presence of different concentrations of butanol, the bacterial strains did not differ in such cytomorphological features as granularity and cell size. The quantitative content of polar and neutral lipids in the presence of butanol varied depending on the content of butanol in the medium, however this effect had a strain-specific character and did not show a correlation with the resistance of these bacteria to butanol. So, the content of polar and neutral lipids varied depending on butanol content in the medium. However this effect was strain-specific independently of resistance of these bacteria to butanol. The use of bacterial biomass as a source of lipids for the production of biofuels requires further optimization of the process to increase the content of the neutral lipid fraction in bacterial cells.
Key words: Clostridium, polar and neutral lipids, butanol, flow cytometry.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2017
References
1. Tkachenko A.F., Tigunova O.A., Shulga S.M. Microbial lipids as a source of biofuel. Cytol. Genet. 2013, 47(6), 343-348. https://doi.org/10.3103/S0095452713060054
2. Guan Z., Tian B., Perfumo A., Goldfine H. The polar lipids of Clostridium psychrophilum, an anaerobic psychrophile. Biochimica et biophysica acta. 2013, 1831(6), 1108-1112.https://doi.org/10.1016/j.bbalip.2013.02.004
3. ohnston N., Goldfine H. Lipid composition in the classification of the butyric acid-producing Clostridia. J. Gen. Microbiol. 1983, 129, 1075-1081. https://doi.org/10.1099/00221287-129-4-1075
4. MacDonald D., Goldfine H. Effects of solvents and alcohols on the polar lipid composition of Clostridium butyricun under conditions of controlled lipid chanecomposition. Appl. Environ. Microbiol. 1991, 57(12), 3517-3521. PMC1840405.
5. Matsumoto M., Tamiya K., Koizumi K. Studies on neutral lipids and a new type of aldehydogenic ethanolamine phospholipid in Clostridium butyricum. J. Biochem. 1971, 69(3), 617-620. https://doi.org/10.1093/oxfordjournals.jbchem.a129508
6. Ogata S., Yoshino S., Okuma Y., Hayashida S. Chemical composition of autoplast membrane of Clostridium saccharoperbutylacetonicum. J. Gen. Appl. Microbiol. 1982, 28, 293-301. Doi.org/10.2323/jgam.28.293 https://doi.org/10.2323/jgam.28.293
7. De la Jara, A., Mendoza, H., Martel, A., Molina, C., Nordstron, L., de la Rosa, V., D?az, R. Flow cytometric determination of lipid content in a marine dinoflagellate, Crypthecodinium cohnii. J. Appl. Phycol. 2003, 15, 433-438. https://doi.org/10.1023/A:1026007902078
8. Wilkinson J.F., The problem of energy-storage compounds in bacteria. Experimental Cell Research. 1959, 7, 111-130. https://doi.org/10.1016/0014-4827(59)90237-X
9. Adler H.I., Crow W. A technique for predicting the solvent-producing ability of Clostridium acetobutylicum. Appl. Environ. Microbiol. 1987, 53(10), 2496–2499. PMC204135.
10. Tracy B.P., Gaida S.M., Papoutsakis E.T. Development and application off-cytometric techniques for analyzing and sorting endospore-forming clostridia. Appl. Environ. Microbiol. 2008, 74(24), 7497–7506. https://doi.org/10.1128/AEM.01626-08
11. Neumeyer A., H?bschmann T., M?ller S., Frunzke J. Monitoring of population dynamics of Corynebacterium glutamicum by multiparameter flow cytometry. Microbial Biotechnology. 2013, 6(2), 157–167. https://doi.org/10.1111/1751-7915.12018
12. Jones S.W., Tracy B.P., Gaida S.M., Papoutsakis E.T. Inactivation of ?F in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes ?E and ?G protein expression but does not block solvent formation. J. Bacteriol. 2011, 193(10), 2429–2440. https://doi.org/10.1128/JB.00088-11
13. Tracy B.P., Jones S.W., Papoutsakis E.T. Inactivation of ?E and ?G in Clostridium acetobutylicum illuminates their roles in clostridial-cell-form biogenesis, granulose synthesis, solventogenesis, and spore morphogenesis. J. Bacteriol. 2011, 193(6), 1414–1426. https://doi.org/10.1128/JB.01380-10
14. Kolek J., Branska B., Drahokoupil M., Patakova P., Melzoch K., Sauer M. Evaluation of viability, metabolic activity and spore quantity in clostridial cultures during ABE fermentation. FEMS Microbiol. Lett. 2016, 363(6): fnw031. https://doi.org/10.1093/femsle/fnw031
15. Skrotska O.I., Penchuk Yu.M., Skrotskuy S.O., Gavrulenko M.M., Smirnova G.F. A selection of acetone-butanol bacteria is from different natural sources. Food Industry, 2010, 9:49-52. URI: dspace.nuft.edu.ua/jspui/handle/123456789/867
16. Kujawska A., Kujawski J., Bryjak M., Kujawski W. ABE fermentation products recovery methods - A review. Renewable and Sustainable Energy Reviews. 2015, 8(8), 648-661. https://doi.org/10.1016/j.rser.2015.04.028