ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 1, 2014
https://doi.org/10.15407/biotech7.01.080
Р. 80-86, Bibliography 25, Ukrainian
Universal Decimal classification: 602.4:537.632: 576.3/.7
SEPARATION OF CELL POPULATIONS BY SUPER-PARAMAGNETIC PARTICLES WITH CONTROLLED SURFACE FUNCTIONALITY
1Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv
2Lviv National Polytechnic University, Ukraine
3Danylo Halytsky National Medical University, Lviv, Ukraine
4Galkin Institute of Physics and Engineering of National Academy of Sciences of Ukraine, Donetsk
The recognition and isolation of specific mammalian cells by the biocompatible polymer coated super-paramagnetic particles with determined surface functionality were studied. The method of synthesis of nanoscaled particles on a core of iron III oxide (Fe2O3, magemit) coated with a polymer shell containing reactive oligoperoxide groups for attachment of ligands is described.
By using the developed superparamagnetic particles functionalized with peanut agglutinin (PNA) we have separated the sub-populations of PNA+ and PNA– cells from ascites of murine Nemeth-Kellner lymphoma.
In another type of experiment, the particles were opsonized with proteins of the fetal calf serum that improved biocompatibility of the particles and their ingestion by cultivated murine macrophages J774.2. Macrophages loaded with the particles were effeciently separated from the particles free cells by using the magnet. Thus, the developed surface functionalized superparamagnetic particles showed to be a versatile tool for cell separation independent on the mode of particles’ binding with cell surface or their engulfment by the targeted cells.
Key words: super-paramagnetic particles, cell selection, NK/Ly lymphoma, macrophages J774.2.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2014
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