ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 1, 2014
https://doi.org/10.15407/biotech7.01.117
Р. 117-122, Bibliography 12, Russian
Universal Decimal classification: 576.3.085.23:577.325
NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES
Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkіv
The morphological and functional characteristics of the cells in cultures in the presence of nanoparticles of Eu2O3 and luminescence characteristics of nanoparticles in cells and also the possible toxic effect of nanoparticles of Eu2O3 in the range of 6.8–340 ?g/ml for SPEV cell line, human fibroblasts in suspension were investigated. The localization of Eu2O3 nanoparticles in SPEV cells and fibroblasts was determined by confocal scanning microscopy.
We found that europium oxide nanoparticles at a concentration of 6.8 ?g/ml did not have a toxic effect on the cell cultures, luminesce in the visible spectral range and remain for a long time in the cells without a significant decrease in intensity of the luminescence. The localization of nanoparticles in cell cytoplasme but not in nuclei was shown by confocal scanning microscopy. They can be used as nanoluminophores for labelling of cell suspensions and cultures.
Key words: SPEV cell line, fibroblasts, nanoparticles, luminophores.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2014
References
1. Ratra Ch.R., Bhattacharya R., Patra S., Basu S., Mukherjee P., Mukhopadhyay D. Lanthanide phosphate nanorods as inorganic fluorescent labels in cell biology research. Clin. Chem. 2007, 53(11), 2029–2031.
https://doi.org/10.1373/clinchem.2007.091207
2. Gao X., Yang L., Petros J. A., Marshall F. F., Simons J. W., Nie S. In vivo molecular and cellular imaging with quantum dots. Curr. Opin. Biotechnol. 2005, V. 16, P. 63–72.
https://doi.org/10.1016/j.copbio.2004.11.003
3. Jaiswal J. K., Mattoussi H., Mauro J. M., Simon S. M. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat. Biotechnol. 2002, V. 21, P. 47–51.
https://doi.org/10.1038/nbt767
4. Freshni R. Culture of animal cells. Мethods. Moscow: Mir. 1989, 333 p. (In Russian).
5. Birger M. O. Manual for microbiological and virological research methods. Medistyna, Moscow. 1982, 462 p. (In Russian).
6. Bazzia R., Flores-Gonzaleza M. A. Synthesis and luminescent properties of sub-5-nm lanthanide oxides nanoparticles. J. Luminesc. 2003, 102(103), 445–450.
https://doi.org/10.1016/S0022-2313(02)00588-4
7. Dyakonov L. P. Living cell in culture. Methods and application in biotechnology. Мoscow. 2000, 355 p. (In Russian).
8. Pavalko F. M., Otey C. A. Role of adhesion molecule cytoplasmic domains in mediating interactions with the cytoskeleton. Proc. Soc. Exp. Biol. Med. 1994, 205(4), 282–293.
https://doi.org/10.3181/00379727-205-43709
9. Pavlovich Е. V., Stepanyuk L. V., Goncharyk Е. I. Features of adhesion and proliferation processes of cell line SPEV in europium oxide nanoparticles incorporating. Nanobiophysics: fundamental and applied aspects (6–9 October 2011, Kyiv). Kyiv. 2011, Р. 106. (In Russian).
10. Bouzigues C., Alexandrou T. G. Biomedical applications of rare-earth based nanoparticles. ACS Nano. 2011, 5(11), 8488–8505.
https://doi.org/10.1021/nn202378b
11. Masson J.-B., Casanova D., T?rkcan S, Voisinne G., Popoff M. R., Vergassola M., Alexandrov A. Interning maps of forces inside cell membrane microdomains, Phys. Rev. Lett. 2009, V. 102, 048103.
https://doi.org/10.1103/PhysRevLett.102.048103
12. Wang L., Li P., Wan L. Luminescent and hydrophilic LaF3-polymer nanocomposite for DNA detection. Luminescence. 2009, 24(1), 39–44.
https://doi.org/10.1002/bio.1061