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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

 1 2014

"Biotechnologia Acta" v. 7, no 1, 2014
https://doi.org/10.15407/biotech7.01.117
Р. 117-122, Bibliography 12, Russian
Universal Decimal classification: 576.3.085.23:577.325

NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES

Pavlovich О. V.

Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkіv

The morphological and functional characteristics of the cells in cultures in the presence of nanoparticles of Eu2O3 and luminescence characteristics of nanoparticles in cells and also the possible toxic effect of nanoparticles of Eu2O3 in the range of 6.8–340 ?g/ml for SPEV cell line, human fibroblasts in suspension were investigated. The localization of Eu2O3 nanoparticles in SPEV cells and fibroblasts was determined by confocal scanning microscopy.

We found that europium oxide nanoparticles at a concentration of 6.8 ?g/ml did not have a toxic effect on the cell cultures, luminesce in the visible spectral range and remain for a long time in the cells without a significant decrease in intensity of the luminescence. The localization of nanoparticles in cell cytoplasme but not in nuclei was shown by confocal scanning microscopy. They can be used as nanoluminophores for labelling of cell suspensions and cultures.

Key words: SPEV cell line, fibroblasts, nanoparticles, luminophores.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2014

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