INFLUENCE OF ETHANOL ON COMPOSITION AND OUTPUT OF BIOMASS OF Dunaliella viridis TEODOR. IN QUASI-CONTINUOUS CULTIVATION Bozhkov A. I., Menzyanova N. G., Sysenko E. I.

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" v. 7, no 1, 2014
https://doi.org/10.15407/biotech7.01.093
Р. 93-99, Bibliography 27, Russian
Universal Decimal classification: 582.232/275.082.26

INFLUENCE OF ETHANOL ON COMPOSITION AND OUTPUT
OF BIOMASS OF Dunaliella viridis TEODOR. IN QUASI-CONTINUOUS CULTIVATION

Bozhkov A. I., Menzyanova N. G., Sysenko E. I.

Research Institute of Biology of Karazin Kharkiv National University, Ukraine

The effect of ethanol (final concentration is 0.3 and 0.5%) on biomass yield and composition (content of nucleic acids, proteins, lipids, and chlorophyll a and b, ?-carotene, and exopolysaccharides) and morphology of the cells Dunaliella viridis in a quasi-mode of cultivation was investigated. It has been found that addition of ethanol in the culture medium to a final concentration of 0.3% increased output 2-fold compared with the quasi-continuous cultivation without alcohol and 2.5 times compared with a cumulative cultivation. Increasing the concentration of ethanol in the medium to 0.5% reduced the biomass yield of Dunaliella viridis compared with 0.3% alcohol concentration. Ethanol increased the output exopolysaccharides into culture medium and changed morphology and Dunaliella viridis motility.

Key words: D. viridis biomass, quasi-continuous cultivation, effect of ethanol.

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