RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS A. A. Samoylenko, N. V. Byts, G. V. Pasichnyk, N. V. Kozlova, A. V. Bazalii, D. S. Gerashchenko, S. G. Shandrenko, A.

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" v. 6, no. 4, 2013
https://doi.org/10.15407/biotech6.04.182
Р. 182-189, Bibliography 15, Ukrainian
Universal Decimal classification: 577.218

RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS

A. A. Samoylenko¹, N. V. Byts¹, G. V. Pasichnyk¹, N. V. Kozlova¹, A. V. Bazalii¹, D. S. Gerashchenko¹, S. G. Shandrenko¹, A. V. Vorotnikov², T. Kietzmann³, S. V. Komisarenko¹, L. B. Drobot¹

¹Palladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine
²Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia
³Department of Biochemistry and Biocenter Oulu, University of Oulu, Oulu, Finland

Ruk/CIN85 is an adaptor protein that plays important roles in the regulation of cellular processes such as cell death, proliferation and motility. It was recently shown that overexpression of Ruk/CIN85 increases the oncogenic potential of human breast adenocarcinoma MCF-7 cells. It was the aim of the present study to investigate whether inhibition of Ruk/CIN85 expression has an effect on the biological properties of the cells. In order to down-regulate Ruk/CIN85 expression of small interfering RNA-based approach was used. For down-regulation of Ruk/CIN85 lentiviral constructs encoding Ruk/CIN85-specific small hairpin RNA sequences were generated. By using the obtained recombinant lentiviruses it was shown that inhibition of Ruk/CIN85 expression influences biological properties (motility, proliferation, ABCG2 expression, and ROS generation) of various tumour cell types such as human breast adenocarcinoma MCF-7, human colorectal adenocarcinoma HT-29, and Lewis mouse lung carcinoma cells.

Key words: adapter proteins, Ruk/CIN85, RNAinterference, lentiviral vectors, cell motility, active oxygen forms.

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