ISSN 1995-5537

"Biotechnology" journal V. 5, No. 2, 2012
Р. 115-121, Bibliography 20, Ukrainian.
Universal Decimal classification: 577.151:579.864.1

ESTIMATION OF AUTOIMMUNE AND DNA-DAMAGE INFLUENCE OF GOLD NANOPARTICLES WITH DIFFERENT SIZES

V. I. Bobyk, L. M. Kapustyan, L. M. Morozova, P. V..Pogribny, S. M. Dybkova,
L. S. Rieznichenko, T. G. Gruzina, Z. R. Ulberg, V. P. Kovalenko, L. L. Sydoryk

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
Ovcharenko Institute of Biocolloidal Chemistry of National Academy of Sciences of Ukraine, Kyiv
Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology
of National Academy of Sciences of Ukraine, Kyiv
SO «Institute of Medicine of Work» of National Academy of Medical Sciences of Ukraine, Kyiv

Water dispersions of gold nanoparticles with average sizes 7, 17 and 57 nm have been synthesized by the method of chemical condensation. The character of autoimmune response of model animals’ organisms induced by intraperitoneal injection of the gold nanoparticles has been studied via the determination of autoantibodies titre against defensin (mBD-2), chaperonin Hsp60 and heart myosin (Myo).

The DNA-damage action of gold nanoparticles with average sizes 7 and 17 nm as well as absence of one under the influence of 57 nm nanoparticles have been revealed in vitro and in vivo.

On the basis of the parameters of organism autoimmune response and DNA-damage influence on tissues of the laborator animals with gold nanoparticles of different size it was made a conclusion that 57 nm particles were the most perspective
for using in technologies of cardiovascular pathologies diagnostic and treatment preparations creation.

Key words: gold nanoparticles, autoantibodies, defensin, chaperonin Hsp60, myosin, immune response, DNA-damage action.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008