ISSN 1995-5537
"Biotechnology" journal V. 4, No. 6, 2011
Р. 18-30, Bibliography 37, Ukrainian.
Universal Decimal classification: 575.24+615.222:581.143.6
Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
A growth and development as a complete organism as a plant tissue culture have determined by different parameters oscillation of the cell system that formed culture lines, tissue or an organism as a whole not only at level processes with circadian rhythms (circadian organization level) but at level processes with passage rhythms during passage organization. However this basic processes have not been adequately explored.
Dynamic of cell population systems in vitro has been studed by the example of Rauwolfia serpentina Benth culture tissues at passage level, specifically temporal organization and robustness of this system. The phase trajectories of productivity indexes dynamics (biomass and indoline alkaloids accumulation), morphogenesis index dynamic (traheid elements dynamic), as well as dynamics of cell part with different DNA content in the nucleus and different nucleoli square at passage level under culturing condition variation for the high-productive Rauwolfia serpentina K-27 strain (ajmaline producer) were researched. The obtained results have indicated point of view that applied biotechnological methods of productivity increasing in the researched cell culture are based on dynamics hereditary memory.
Key words: cell population in vitro, dynamic of the cell systens, phase trajectories, Rauwolfia serpentina.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008