"Biotechnology" journal V. 4, No. 5, 2011
Р. 45-56, Bibliography 20, Ukrainian.
Universal Decimal classification: 577.112.384:612.815.1

INTERACTION OF POLIMER-COVERED NANOPARTICLES OF MAGNETITE WITH BRAIN NERVE TERMINALS AND BLOOD PLATELETS

Т. O. Borisova¹, N. O. Dudchenko², O. B. Brik², L. O. Каsatkina^{1, 3}, R. V. Sivko¹, O. Yu. Chunikhin¹, N. V. Krisanova¹

¹Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv;
²Semenenko Institute of Geochemistry, Mineralogy and Ore Formation of National Academy of Sciences of Ukraine, Kyiv;
³Educational and Scientific Center «Institute of Biology», Taras Shevchenko National University

In nanoneurotechnology, usage of magnetic nanoparticles is a very perspective way for transport of active substances and drugs to target cells in response to external manipulation and anticancer therapy. Interaction of synthesized nanoparticles of magnetite covered with dextrane, hydroxyethyl starch, oxidized hydroxyethyl starch, chitozan and silica with brain nerve terminals and blood platelets (as peripheral model of presynapse) was analyzed. The binding of nanoparticles with nerve terminals and platelets was demonstrated using photon correlation spectroscopy and flow cytometry. Using potential-sensitive fluorescent dye rhodamine 6 G, we showed that nanoparticles did not influence the potential of the plasma membrane of nerve terminals and platelets. Acidification of synaptic vesicles of nerve terminals and secretory granules of platelets was not changed in the presence of nanoparticles that was revealed with pH_sensitive fluorescent dye acridine orange.

Key words: magnetic nanoparticles, synaptosomes, blood platelets.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008