ISSN 1995-5537
"Biotechnology" journal V. 4, No. 4, 2011
Р. 87-94, Bibliography 24, Russian.
Universal Decimal classification: 581.143.6
L. E. Sergeeva, L. I. Bronnikova, E. N. Tishchenko
Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine, Kуіv
Problem of plant resistance to stress is one of the most fundamental and is a scope of researches on all hierarchical levels. Currently, it has become more urgent due to the depletion of wild and cultivated flora caused by violations (often irreversible) of ambient and environmental conditions.
Cd-resistant cell lines of tobacco (Nicotiana tabacum L.) S. No. 1, and S. No. 6 were affected by cadmium ions, sea water salts, sodium sulfate and mannitol in lethal dozes. These lines went on growing under pressure of any stress substance. Relative mass growth of calli was the greatest during cultivation on selective media with the addition of Cd2+-ions and exceeded this parameter measured at normal conditions. Free proline contents was investigated in periods of cell active development. During cultivation on all the selective media, free proline levels of the cell lines S. No. 1 exceeded benchmarks. Proline level was smaller than test data when S. No. 6 lines were cultivated on medium containing Na2S04. Peaks of the free proline contents were in both cell lines during their development on mannitolcontaining medium. Free proline content fluctuation could be an indicator of an organism vital factor under stress conditions. The concept about different adaptation of Cd-resistant lines of tobacco to alinity is suggested.
Key words: N. tabacum L., cell lines, cadmium ions, osmotic stress, resistance.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008