ISSN 1995-5537
"Biotechnology" journal V. 4, No. 4, 2011
Р. 34-49, Bibliography 22, Russian.
Universal Decimal classification: 577.113.4:546.28:546.212
HYDRATED PROPERTIES OF COMPOSITE MATERIAL
BASED ON NANOSILICA AND DNA
V. V. Turov, V. M. Barvinchenko, T. V. Krupska, V. M. Gun’ko, V. F. Chekhun
Chuiko Institute of Surface Chemistry of the National Academy of Sciences of Ukraine, Kyiv
Kavetski Institute of Experimental Pathology, Oncology and Radiobiology
of the National Academy of Sciences of Ukraine, Kyiv
Silica is widely used in biomedicine not only as concomitant matter giving dosage forms the required physical and chemical properties but also as independent medication with the expressed detoxicated effect proved itself to be active at treatment of the food poisonings, bacterial and wound infections.
A nanocomposite system was prepared with finely dispersed silica modified by adsorbed DNA. Nanocomposite properties were studied using NMR spectroscopy, thermogravimetry, and infrared spectroscopy. A biometric study of SiO2-DNA composite influence was carried out for suspended Saccharomyces cerevisiae yeast cells. It was shown that the composite possesses a significant bioactivity since it can substantially accelerate the processes of vital functions of bakery yeasts. Hydration of SiO2-DNA composite and influence of organic solvent media were studied in detail. It was found out some forms of interfacial water including weakly associated water that practically does not form the hydrogen bonds with neighboring molecules. It was shown that interconversion of different forms of interfacial water occurs at varying temperature or addition of organic compounds. It was assumed that weakly associated water could affect the biocompatibility of nanoparticles with respect to cells.
Key words: Silica-DNA nanocomposite, hydratation, strongly and weakly associated water, suspension of Saccharomyces cerevisiae cells.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008