ISSN 1995-5537
"Biotechnology" journal Vol. 4, No. 1, 2011
Р. 101-105, Bibliography 22, English.
Universal Decimal Classification: 576.314+576.367+576.385
APOPTOSIS-DEPENDENT CHANGES IN EXPRESSION PATTERN
OF mRNA CODING FOR SIALYL-MODIFYING ENZYMES
Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv
mRNA expression levels of seven major cellular sialyl-modifying enzymes (Neu1, Neu2, Neu3, Neu4 sialidases and hST6Gal.I, hST3Gal.III, hST3Gal.IV sialyltransferases) during apoptotic cells death was compared. Identification of enzymes responsible for the processes of superficial desialylation at an apoptosis can become a potential biotechnology for modifying of AC phagocytosis and treatment of autoimmune disorders. Reverse-type PCR analysis to evaluate mRNA expression in human Jurkat T leukemia cells was applied. After 2 h of etoposide-induced apoptosis (when cells were still annexin V-negative), an increased mRNA expression of Neu1, Neu3, Neu4, hST6Gal.I and hST3Gal.IV, and decreased expression of hST3Gal.III mRNA were found. Expression mRNA of the above mentioned enzymes was decreased after 6 and 12 hour of apoptosis induction, when cells gained typical apoptotic characteristics. mRNA level of Neu2 was not significantly changed during the course of apoptosis. Increase of sialidase activity during early steps of apoptosis and decrease afterwards were confirmed by enzymatic fluorometric assay using 2′-(4-methylumbelliferyl)-α-D-N-acetylneuralminic acid as substrate.
Key words: apoptosis, sialylation, neuraminidase, sialyltransferase, mRNA, glycoprotein.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008