DIRECT ORGANOGENESIS IN VITRO OF Gentiana lutea L. I. I. Konvalyuk, N. B. Kravets, N. M. Drobyk, V. M. Mel’nyk, V. A. Kunakh

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ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 5, 2010
Р. 66-73, Bibliography 12, Ukrainian.
Universal Decimal classification: 576.5: 582.923.1

DIRECT ORGANOGENESIS IN VITRO OF Gentiana lutea L.

I. I. Konvalyuk, N. B. Kravets, N. M. Drobyk, V. M. Mel’nyk, V. A. Kunakh

Institute of Molecular Biology and Genetics of National Academy of Sciencies of Ukraine, Kyiv;
Volodymyr Hnatiuk Ternopil National Pedagogical University

Adventitious shoots and roots through the direct organogenesis in vitro from the explants of stem and root origin have been generated for plants derived from four G. lutea populations. The efficiency of regeneration was found to vary with the genotype of maternal plant, kind of explants and phytohormone content and ratio in the nutrient medium. It was revealed that to generate the regenerants from the stem and root explants the most effective was combination of 5 to 10 mg/l thidiazuron with 0.01 or 1.0 mg/l 1-naphtaleneacetic acid in the Murashige-Skoog nutrient medium. The leaf explants failed to produce regenerants at all. The regeneration indices for root explants exceeded those for stem ones several times.

Key words: Gentiana lutea L., regeneration in vitro, rhizogenesis, shoot regeneration.