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ISSN 1995-5537

Cover 5 2010

"Biotechnology" journal Vol. 3, No. 5, 2010
Р. 74-82, Bibliography 31, Russian.
Universal Decimal classification: 575.827:604.6:582.683.2

CREATION OF TRANSFORMED RAPE PLANTS THAT EXPRESS CYP11A1 CYTOCHROME P450 GENE
OF ANIMAL ORIGIN

 L. О. Sakhno, B. V. Morgun, О. Yu. Kvasko, М. V. Kuchuk

Institute of Cell Biology and Genetic Engineering of National Academy of Sciences of Ukraine, Kyiv

The transgenic rape plants were obtained as a result of Agrobacterium tumefaciens-mediated transformation of leaf disks with gene constructs carrying cyp11A1 cytochrome Р450SCC gene from bovine adrenal cortex mitochondria. They are characterized by changes in the accumulation of total soluble protein, increase of antioxidant activity, and reduction of vegetative development period.

Key words: Brassica napus, cyp11A1gene, genetic transformation, cytochrome Р450SCC, antioxidant activity.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008