ISSN 1995-5537
"Biotechnology" journal Vol. 3, No. 3, 2010
Р. 42-49, Bibliography 41, Ukrainian.
Universal Decimal classification: 577.112.7:616
D. O. Minchenko, I. V. Bozhko, T. O. Zinchenko, V. G. Mykhalchenko,
О. P. Yavorovsky, O. G.Minchenko
Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv;
National O.O. Bogomoletz Medical University, Kyiv
Physiological and biochemical processes in organisms are circadian. Disturbance of regulation of circadian processes leads to developing of different pathology and cancer in particular. The genes of circadian factors Per1, Per2, Clock and BMal1 as well as caseinkinase-1 are important regulators which control these processes in normal and pathological conditions. We have shown that the expression of caseinkinase-1, BMal1, Per1, Per2 and Clock as well as 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4 (PFKFB-4) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) genes significantly destroyed in the brain of rats treated with silver nanoparticles: expression of some genes increased, but other — decreased. It was shown that changes in the expression of circadian genes and caseinkinase-1 were observed in a day after treatment of rats with silver nanoparticles but in 3 and 14 days these changes were more expressed. Disturbance of caseinkinase-1 and circadian genes expression could destroy the cellular signal pathways in brain and lead to developing of pathological processes. Results of this investigation demonstrated that silver nanoparticles had significant effect on important regulatory mechanisms controlling metabolic processes in brain cells via circadian gene expression. Expression of circadian genes could be possible a sensitive test for detection of silver nanoparticles action on the organisms.
Key words: silver nanoparticles, gene expression, casein kinase-1 , Per1, Per2, Clock, BMal1, PFKFB-4, brain, rats.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
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