ISSN 1995-5537
«Biotechnology» journal, Vol. 3, No. 2, 2010
Р. 71-81, Bibliography. 21, Ukrainian.
Universal Decimal classification: 577.151.4 +543.554.2 + 544.475
Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
Institute of Semiconductor Physics of National Academy of Sciences of Ukraine, Kyiv
Potentiometric multibiosensor with variety of immobilized enzymes as bioselective elements and a matrix of pH-sensitive field effect transistors as transducers of biochemical signal into an electric one were used in the work. At first inhibition influence of separate toxins and their mixture on bioselective elements of the multibiosensors was studied. The results obtained with the multibiosensors were mathematically processed. A new procedure of multibiosensor analysis was proposed.Using this procedure, the the experiments on determination of toxins in water solutions were carried out.
Series of experiments of toxic compounds determination in water samples of different origin were done by using developed analyzer. The obtained results were compared with ones obtained by standard traditional methods of toxic agents analysis (atomic absorption spectroscopy, thin-film chromatography, and atomic absorption analyzer of mercury). Correlation of the results obtained with the multibiosensor and traditional methods was shown.
Key words: multibiosensor, pH-sensitive field-effect transistors, enzymes, inhibitory analysis, pesticide, ions of heavy metal.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008