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ISSN 1995-5537

3  2008

“Biotechnology” journal Vol. 1, No. 3, 2008
Р. 37-46, Bibliography 29, Ukrainian.
Universal Decimal classification 577.112.083:577.112.4:577.151.05

Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv
Lviv Regional Perinatal Center

Analysis of antigen specificity, catalytic and cytotoxic activity of colostrum immunoglobulins was carried out in order to detect novel molecular markers of autoimmune disorders. Immunoglobulins were isolated from parturient women colostrums by precipitation with 50% ammonium sulfate. It was shown that preparations of immunoglobulins possessed significantly different cytotoxic activity towards Jurkat leukemia T-cells, various hydrolyzing ability towards plasmid DNA and histone H1, and also differed by content of auto-antibodies to doublestranded DNA and histones. We concluded that functional properties of colostrums immunoglobulins depended on individual peculiarities of parturient women humoral immunity. These properties can be used for complex detection of early autoimmune disorders linked with pregnancy and delivery.

Key words: colostrum of healthy women, immunoglobulins, cytotoxic activity, catalytic activity, level of auto-AB.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008