ISSN 1995-5537

“Biotechnology” journal Vol. 1, No.1, 2008
P. 116-122, Bibliography. 20, Ukrainian
Universal Decimal classification: 577.15.543.555

CONDUCTOMETRIC BIOSENSOR BASED ON THE THREENZYME SYSTEM FOR DETERMINATION OF SUCROSE

Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv
National Taras Shevchenko University, Kyiv

In the course of this study, a biosensor for sucrose determination, with a complex threeenzyme membrane representing a sensitive element immobilized onto a conductometric transducer, has been created. The time of determining sucrose concentration in the solution is 1–2 minutes. The dynamic range of determination may vary depending on buffer capacity and amounts to 2 ?M — 5 mM of sucrose in 5 mM of phosphate buffer solution.

In this work the dependence on pH and ionic strength of the solution has been analyzed.The developed conductometric biosensor is characterized by high operational stability and repeatability of the response. The data on sensor selectivity and its storage in different conditions are presented as well. The sensor turned out to be suitable for usage in real-life environment.

Key words: sucrose, glucose, conductometric biosensor, invertase, mutarotase, glucoseoxidase.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008