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ISSN 2410-7751 (Print)
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Biotechnologia Acta Т. 18, No. 2, 2025
P. , Bibliography 3 , Engl.
UDC 543.06:577.15:543.553
LABORATORY PROTOTYPE OF ELECTROCHEMICAL BIOSENSOR FOR THE QUANTITATIVE ASSESSMENT OF ALANINE AMINOTRANSFERASE ACTIVITY
D.O. MRUGA *1, Y.R. VAKHOVSKY 1,2, S.V. DZYADEVYCH1,2, O.O. SOLDATKIN 1,3
1 Institute of Molecular Biology and Genetics, NAS of Ukraine, 150, Akademika Zabolotnoho St, Kyiv 03680, Ukraine
2 Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Street, Kyiv 01601, Ukraine
3 National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute", 37, Beresteyskyi Prospect, Kyiv, 03056, Ukraine
Aim: To develop an electrochemical biosensor for the quantitative assessment of alanine aminotransferase (ALT) activity.
Materials and Methods. Three-electrode amperometric scheme of detection with platinum disk working electrode covered with bioselective element based on pyruvate oxidase and photopolymer. Measurements were carried out with an applied potential of 0.6 V.
Results. A laboratory prototype of an amperometric biosensor for ALT detection was developed. The immobilization method was selected, and the procedure was optimized for the type of bioselective material used. The composition of the working buffer was optimized, namely, the kind of buffer, buffer capacity, pH, content of coenzymes of the bioselective material (phosphate ions, magnesium ions, thiamine pyrophosphate), and the analyte (alanine, ketoglutarate, pyridoxal phosphate). The reproducibility of immobilization and reproducibility of responses of the developed biosensor, as well as its analytical characteristics (linear range, detection limit, response time, etc.), were analyzed.
Conclusions. The developed biosensor was characterized by sufficiently good analytical parameters for its further optimization and testing when working with real blood serum samples.
Keywords. Alanine aminotransferase, biosensor, enzyme activity
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2025
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ISSN 2410-7751 (Print)
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Biotechnologia Acta Т. 17, No. 2 , 2024
P. , Bibliography , Engl.
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Selective sodium pump inhibitor Calix[4]arene C-1130 increases concentration of Ca ions in smooth muscle cells
Melnyk V.V., Malyuk O.V., Veklich T.O.
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Biotechnologia Acta
DOI of the journal: https://doi.org/10.15407/biotech
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ISSN 2410-7751 (Print)
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Biotechnologia Acta is a multidisciplinary blind peer-reviewed scientific journal, published by the Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.
Topics of interest include, but are not limited to:
— molecular biotechnology
— nanobiotechnology
— immunobiotechnology
— biosensory and molecular diagnostics
— biotechnology of biologically active substances obtaining
— biotechnology of transgenic organisms
— biotechnology for food and food contaminants
— stem cells and gene therapy
— bioengineering and engineering enzymology
— biotechnology and health
— agrobiotechnology
— biodegradation and recycling
— legal and ethical aspects of biotechnology.
The journal was founded in 2005, registered under Біотехнологія name and was renamed in 2013, КВ No 19650-9450ПР series at 01.30.2013, as Biotechnologia Acta, the official abbreviation is Biotechnol. Acta. It is transliterated as Biotekhnolohiia.
Biotechnologia Acta is issued bimonthly, publication is free-of-charge.
The format of Biotechnologia Acta corresponds to the international standards.
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