ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 2 , 2024
P. 76-77, Bibliography 6, Engl.
UDC:: 577.2:616-006, 611.013, 616-006, 616-006.04
DOI: https://doi.org/10.15407/biotech17.02.076
EXPRESSION PATTERN OF THE MRPS18 FAMILY PROTEINS IN CHORDOMA
A.V. SUSHNOVA 1, L.M. KOVALEVSKA 1, E.V. KASHUBA 1, T.A. MALYSHEVA 2
1 RE Kavetsky Institute of experimental pathology of the National Academy of Sciences of Ukraine, Kyiv;
2 The State Institution Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine, Kyiv
Chordomas are rare tumors of the axial skeleton that arise from the notochord; they are usually observed at the skull's base and rarer along the spine. Chordomas resist to conventional chemotherapy, so finding new diagnostic and/or prognostic markers is essential. We have recently shown that genes of the mitochondrial ribosomal protein S18 (MRPS18) family are differentially expressed in gliomas. The MRPS18-2 oncoprotein is expressed higher in cancerous cells than its standard counterpart.
Aim. We wanted to investigate the peculiarities of the expression pattern of the MRPS18 family genes in chordoma to understand their role in cancerogenesis better, using an immunohistochemical analysis on chordoma clinical samples.
Methods. 15 specimens of chordoma of the base of the skull and 5 samples of the spine localization were studied retrospectively. The specific antibodies against MRPS18-1, MRPS18-2, MRPS18-3, and RB were used for an immunohistochemical analysis. All cases were stained in parallel with appropriate negative control.
Results. We found that MRPS18 family proteins are differentially expressed in chordoma tissues. MRPS18-1 showed the strongest signal in all tumor samples. The lowest level of signal intensity was recorded for the MRPS18-3 protein. In several samples, the RB signal was detected in the cytoplasm of tumor cells. Notably, the intense staining for MRPS18-2 was also observed in these samples. The delicate mechanism of sequestering of RB in the cytoplasm of cancer cells should be further elucidated.
Conclusion. These preliminary results should lead to more extensive work on the role of RB-MRPS18 family proteins interaction for chordoma development.
Key words: chordoma, MRPS18 family genes, proteins MRPS18-1, MRPS18-2 and MRPS18-3, RB gene, RB protein.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024