ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 2 , 2024
P. 59-61, Bibliography 5, Engl.
UDC::616.36-004+340+612.017.1
DOI:https://doi.org/10.15407/biotech17.02.059
DONOR-SPECIFIC ANTIBODIES AS A PREDICTOR OF GRAFT REJECTION AFTER LIVER TRANSPLANTATION
A.V. Kukhol, N.A. Tsokolenko, A.O. Mazanova, Y.A. Hrohul
National Specialized Children's Hospital “OHMATDYT” of the Ministry of Health of Ukraine, Kyiv
The main reason for graft loss is the rejection of the donor organ, which may occur at different times after transplantation and may be caused by the recipient's organism reaction against the donor's human leukocyte antigen (HLA) proteins. Donor-specific antibodies (DSA) are produced in the patient’s organism as a response to foreign HLA antigens.
Aim. The purpose of our study was to evaluate the effects of already existing and/or de novo generated DSAs in liver transplantation as predictors of graft rejection and to establish an interconnection between blood biochemical parameters (Alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin level) with the level of DSA in patients with liver transplant.
Methods. xMAP-Luminex next-generation flow cytometry technology and LABScreen Single antigen beads reagent (Onelambda, USA) were used for anti-HLA determination. Total bilirubin level was detected photometrically. The activity of ALT and AST was determined spectrophotometrically on the automatic analyzer COBAS C 111 (Roche, Switzerland) by the manufacturer's instruction.
Results. Detection of DSA and PRA was necessary at the same level as a measurement of classical biochemical parameters of liver function (ALT, AST, etc.) for monitoring graft status and prevention of acute or chronic rejection and choosing the correct immunosuppression protocol.
Conclusions. The DSA and PRA levels, as well as total bilirubin and ALT and AST activity, corresponded to each other and could be used for comprehensive both pre- and post-transplantation screening of patients requiring liver transplantation or re-transplantation. Detection of DSA and PRA was necessary at the same level as a measurement of classical biochemical parameters of liver function (ALT, AST, etc.) for monitoring graft status and prevention of acute or chronic rejection and choosing the correct immunosuppression protocol.
Key words: HLA antibodies, donor-specific antibodies, transplantation screening.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024