ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 2 , 2024
P. 41-43, Bibliography 4, Engl.
UDC:: 577.332.4
DOI: https://doi.org/10.15407/biotech17.02.041
CALIX[4]ARENES С-715 AND С-772 AS INSTRUMENTS OF INFLUENCE ON Са2+-TRANSPORT AND BIOENERGETICS IN MYOMETRIAL MITOCHONDRIA
I.S. Forys, M.R. Pavliuk
Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv.
Aim. The goal of this work is to study the effects of calix[4]arenes C-715 and C-772 on Ca2+-transport, the electron transport chain activity, and the reactive oxygen species generation in the mitochondria of rat uterine smooth muscle.
Methods. Measurement of changes in NADH autofluorescence, as well as the energy-dependent accumulation of Ca2+ and reactive oxygen species generation in the fraction of isolated mitochondria, was carried out using the spectrofluorimetry method. The hydrodynamic diameter of mitochondria was measured using the laser correlation spectroscopy method.
Results. It was found that calix[4]arenes C-715 and C-772 moderately inhibit both the NADH oxidation and the energy-dependent Ca2+ accumulation by isolated mitochondria.We also showed that depending on concentration and time, the studied calix[4]arenes reduce the level of reactive oxygen species generation by mitochondria. Additionally, it was revealed that the studied compounds moderately increase mitochondria hydrodynamic diameter depending on concentration.
Conclusions. Studied calix[4]arenes slow the oxidation of NADH in isolated mitochondria, which represents an inhibitory effect on the electron transport chain functioning, in particular its complex I. Since this might lower the inner mitochondrial membrane's electrochemical potential, we observed reduced energy-dependent Ca2+ accumulation. Selected compounds decrease reactive oxygen species generation by isolated mitochondria, which have a protective effect on organelles. A moderate increase in mitochondria hydrodynamic diameter suggests that the studied compounds do not cause mitochondrial dysfunction. Researched calix[4]arenes can be used in experimental practice to influence the mitochondrial functional activity.
Key words: calix[4]arene, Ca2+, bioenergetics, mitochondria, myometrium.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024