ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 2 , 2024
P. 70-73, Bibliography 8, Engl.
UDC:: 577
DOI: https://doi.org/10.15407/biotech17.02.070
ANTICOAGULANT PROPERTIES OF RECOMBINANT ANNEXIN А5
D. O. SAVCHENKO 1,2, A. A. SIROMOLOT 1,3, A. O. PAVLENKO 1
1 Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv
2 National University “Kyiv-Mohyla Academy”, Kyiv, Ukraine
3 Taras Shevchenko National University of Kyiv, Ukraine
Aim: Because of its involvement in membrane properties, annexin A5 has a significant impact on membrane-dependent processes within hemostasis, including the initiation of the coagulation cascade, the activation of factor X and prothrombin, and the function of protein C on membrane surfaces. By binding to phosphatidylserine, annexin A5 acts as a protective shield, masking exposed phospholipid surfaces and exerting a general anticoagulant effect. Considering the significant influence of annexin A5 on the activation of hemostatic compounds on cell surfaces, it is crucial to investigate its mechanisms further.
Methods: After purification of polyHis-Tag proteins by immobilized metal affinity chromatography from E. coli Rosetta cell biomass, we checked the purity of the eluate by SDS-PAGE. We performed the APTT, PT, and platelet aggregation to determine the anticoagulation influence of recombinant annexin A5 in these tests.
Results: Annexin in the highest concentration of 40 μg/ml prolonged blood plasma clotting time in activated partial thrombin time by almost 50%, blood plasma clotting time in prothrombin time by nearly 15% and did not influence platelet aggregation rate..
Conclusion: It can be assumed that its anticoagulant effect is directed to the enzyme complexes of the hemostasis system and does not extend to platelet receptors. The obtained protein can be used to study the activation of hemostatic components on cell surfaces.
Key words: annexin A5, blood coagulation, platelet aggregation, phosphatidylserine, platelets.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024