ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta  V. 15, No. 4, 2022
P. 27-29. Bibliography 5, Engl.
UDC: 577.175
https://doi.org/10.15407/biotech15.04.027

CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIES

D.I.Ostapchenko¹, N.P.Vedenicheva ², L.V.Garmanchuk¹

¹Taras Shevchenko National University of Kyiv "Institute of Biology and Medicine", Ukraine
² Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv

Aim. This study was aimed to examine influence of сytokinin fraction of basidiomycete Hericium coralloides on the spontaneous and induced phagocytic activity of murine peritoneal macrophages.

Materials and methods. Mononuclear phagocyte (macrophage) fraction of peritoneal exudate of C57BL/6 mice was used. Macrophages were incubated under standard conditions at 37 °C, 100% humidity and 5% CO₂ for 4 hours. Phorbol 12-myristate 13-acetate (PMA) was added to part of the samples to activate oxidative metabolism. Hericium coralloides was added  in two concentrations that were 5 and 10 times lower than the IC50, defined as an antiproliferative effect on colon cancer cells. Incubation with samples was carried out for 2 hours.

Results. When adding PMA, Hericium coralloides (0.017 and 0.035 ?g/ml) and in the combination of PMA with Hericium coralloides, activation of reactive oxygen species (ROS) in peritoneal macrophages was revealed by 1.37-1.7 times, compared to the spontaneous activity of phagocytes.

Conclusions. Thus, the effect of the cytokinin e.tract of the basidium fungus Hericium coralloides was manifested by an increase in the phagocytic activity of peritoneal macrophages as one of the possible mechanisms of immunomodulatory action.

Key words: Hericium coralloides, NBT test, oxidative metabolism, peritoneal macrophages

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2022