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ISSN 1995-5537

2 2010

"Biotechnology" journal Vol. 3, No. 2, 2010
Р. 9-18, Bibliography. 77, Russian
Universal Decimal classification: 575.827:604.6:582.683.2

 
Institute of Cell Biology and Genetic Engineering of National Academyof Sciences of Ukraine, Kyiv
 

Achievements and prospects of creation and application of oilseed cruciferous plants (rapeseed Brassica napus L. var oleifera DC., Indian mustard Brassica juncea (L.) Czern, Abyssinianmustard Brassica carinata A. Braun) for obtaining of unsaturated fatty acids with a long (C18-C24) carbon chain for dietary (stearidonic, arachidonic, eicosapentaenoic, docosahexaenoic, nervonic), pharmaceutical (γ-linolenic, taxoleic, pinolenic) and technical (erucic, gondoinoic, dokozadienoic) purposes are discussed.

Key words: Brassica napus, Brassica juncea, Brassica carinata, monounsaturated fatty acid, polyunsaturated fatty acids.

© Институт биохимии им. А. В. Палладина НАН Украины, 2008

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ISSN 1995-5537

2 2010

«Biotechnology» journal, Vol. 3, No. 2, 2010
Р. 89-95, Bibliography. 15, Russian..
Universal Decimal classification: 615.32.451.16 + 638.178


Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkіv;
Institute of Chemistry at V. N. Karazin, Kharkіv National University, Kharkіv
Chemical composition of an extract derived from dead bee bodies (apiextract) was studied. The extraction procedure was optimized, chemical composition and antioxidant activity depending on the extraction conditions were estimated. It has been shown that apiextract is a valuable source of biologically active compounds: vitamins, amino acids, and antioxidants. Antioxidant activity of the apiextract is highly competitive to ones of traditional pharmaceutical preparations. It was established that the maximum extract yield is achieved after 25–30 extraction cycles when using 150 ml ethanol per 10 g of raw material, moreover purification of both initial raw material and the extract itself from admixtures results in a reduction of its biological activity, therefore it is not desirable.
 

Key words: extraction, dead bee bodies, apiextract, antioxidant activity.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2010
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ISSN 1995-5537

2 2010

«Biotechnology» journal, Vol. 3, No. 2, 2010

С. 82 – 88, Bibliography. 19, Russian..
Universal Decimal classification: 636.5.082.453
 
Poultry Research Institute of the Ukraine Academy of Agrarian Sciences, Borki,
Zmiev District of Kharkіv Region
Three types of the cells for production of avian germ line chimeras: blastodermal, blood and gonad cells were used. Blastodermal cells were isolated from freshly-laid eggs, blood and gonad cells — from embryos of 13-14 and 28 stages of development respectively and were cultured for 15 days. For the first time busulfan as an agent suppressing proliferation of host primordial germ cells was used for production of avian chimeras at blastoderm stage. Only in the case of blastoderm cells using germ line chimeras were obtained. The method allows germ line chimeras production at the level of 70% and expressiveness of the property varies from 4 to 61%.
 

Key words: bird, blastodermal cells, primordial germ cells, chimeras, busulfan.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
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ISSN 1995-5537

2 2010

«Biotechnology» journal, Vol. 3, No. 2, 2010
Р. 71-81, Bibliography. 21, Ukrainian.
Universal Decimal classification: 577.151.4 +543.554.2 + 544.475


Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
Institute of Semiconductor Physics of National Academy of Sciences of Ukraine, Kyiv

Potentiometric multibiosensor with variety of immobilized enzymes as bioselective elements and a matrix of pH-sensitive field effect transistors as transducers of biochemical signal into an electric one were used in the work. At first inhibition influence of separate toxins and their mixture on bioselective elements of the multibiosensors was studied. The results obtained with the multibiosensors were mathematically processed. A new procedure of multibiosensor analysis was proposed.Using this procedure, the the experiments on determination of toxins in water solutions were carried out.
Series of experiments of toxic compounds determination in water samples of different origin were done by using developed analyzer. The obtained results were compared with ones obtained by standard traditional methods of toxic agents analysis (atomic absorption spectroscopy, thin-film chromatography, and atomic absorption analyzer of mercury). Correlation of the results obtained with the multibiosensor and traditional methods was shown.

Key words: multibiosensor, pH-sensitive field-effect transistors, enzymes, inhibitory analysis, pesticide, ions of heavy metal.
 
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
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ISSN 1995-5537

2 2010

«Biotechnology» journal, Vol. 3, No. 2, 2010
Р. 62 - 70, Bibliography. 35,  Russian.
Universal Decimal classification: 576.371:57.083

 
Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv
 
Device and conditions for alginate microbead generation are described. Methods for cell viability evaluation in alginate hydrogel are discussed. Encapsulation procedure was shown to have no significant influence on mesenchymal stromal cell (MSC) viability. It was found that the cells cultivated in alginate microbeads during 8 days maintained viability and metabolic activity however it was accompanied with proliferation stoppage. MSC released from alginate hydrogel were able to adhere to cultural plastic and renew proliferation. The results obtained allow to consider alginate hydrogel as biocompatible material for encapsulation of MSC. Therefore MSC encapsulated in alginate microbeads can be applied to cell technologies and tissue engineering.

Key words: alginate, alginate microbeads, encapsulation, mesenchymal stromal cells, cultivation.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
  1. INFLUENCE OF POLYHEXAMETHYLENEGUANIDINE HYDROCHLORIDE ON THE CHICKEN EMBRYOS FIBROBLASTS PLASMATIC MEMBRANE AND ARTIFICIAL BYLAYER LIPID MEMBRANE A. V. Lysytsya, P. Y. Kryvoshya, O. Ya. Shatursky
  2. SCREENING OF MICROMYCETES AS PRODUCERS OF INULINASE V. I. Stoiko, N. M. Zhdanova, V. L. Aisenberg, A. P. Kapichon
  3. OBTAINING OF MUTANTS OF Вacillus sp. WITH ENHANCED ELASTASE PRODUCTION Matselyukh O. V.
  4. ACTIVITY OF NATIVE AND MODIFIED LIPOPOLYSACCHARIDES OF Rahnella aquatilis L. D. Varbanets, L. B. Skoklyuk, V. V. Shubchynskyy, I. I. Seyfullina, N. V. Shmatkova, S. I. Pokhyl