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ISSN 1995-5537
CONTENT
“Biotechnology” journal Vol. 1, No.1, 2008
P. 116-122, Bibliography 20, Ukrainian.
Universal Decimal classification577.15.543.555
CONDUCTOMETRIC BIOSENSOR BASED ON THE THREENZYME SYSTEM FOR DETERMINATION OF SUCROSE
O. O. Soldatkin, V. M. Peshkova, S. V. Dzyadevych, H. V. Elska
Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
National Taras Shevchenko University, Kyiv
In the course of this study, a biosensor for sucrose determination, with a complex threeenzyme membrane representing a sensitive element immobilized onto a conductometric transducer, has been created. The time of determining sucrose concentration in the solution is 1–2 minutes. The dynamic range of determination may vary depending on buffer capacity and amounts to 2 ?M — 5 mM of sucrose in 5 mM of phosphate buffer solution. In this work the dependence on pH and ionic strength of the solution has been analyzed.The developed conductometric biosensor is characterized by high operational stability and repeatability of the response. The data on sensor selectivity and its storage in different conditions are presented as well. The sensor turned out to be suitable for usage in real-life environment.
Key words: sucrose, glucose, conductometric biosensor, invertase, mutarotase, glucoseoxidase.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
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ISSN 1995-5537
“Biotechnology” journal Vol. 1, No. 1, 2008
P. 106-115, Bibliography. 60, Russian.
Universal Decimal classification: 612.017:543.05
ANALYSIS MYCOTOXINS: PREPARATION OF SAMPLES
M. F. Starodub, L. M. Pilipenko, O. V. Egorova, I. V. Pilipenko, O. S. Gojster, G. O. Chmel’nitsky
Palladin Institute of Biochemistry of Nationale Academy of Sciences of Ukraine, Kyiv
Odessa National Academy of food technologies
National Agricultural University, Kyiv
Analysis of main principles of selection of samples and their preparation for the determination of mycotoxins content in different environment objects is presented. The main attention was addresed to trichothecenes mycotoxins and zearalenone, aflatoxin and fumonisins.
Kеy words: mycotoxins, analysis, selection and preparation of the samples.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
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ISSN 1995-5537
“Biotechnology” journal Т. 1, № 1, 2008
P. 100-105, Bibliography. 5, Ukraininan.
Universal Decimal classification: 616.36:578.891
Highly effective technology for production of recombinant analogue of p24 protein of bovine leukemia virus with proven antigenic properties using immunoenzyme was developed. During investigation the best strain E. coli BL21(DE3) CodonPlus-RIL for introduction of recombinant plasmid was chosen, the composition of growth media was optimized. The procedure of metal-helate-affinity chromatography and refolding of recombinant protein was developed. The results of immunoenzyme were used as criteria of quality of recombinant protein on each stage of the work. The influence of bacterial host strains and growth media composition on the yield and distribution of recombinant protein between soluble form in cytoplasm and insoluble inclusion bodies was shown. Using this technology it is possible to obtain recombinant protein with 98% purity and improved antigenic properties. This protein can be used to increase specificity and sensitivity of BLV-diagnostic test kits based on results of immunoenzyme.
Key words: bovine leukemia virus, protein p24, Escherichia coli BL21 (DE3), metal-helate affinity chromatography, refolding, enzyme-linked immunosorbent assay
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ISSN 1995-5537
“Biotechnology” journal Vol. 1, No. 1, 2008
P. 86-99, Bibliography. 18, English
Universal Decimal classification: 541.64:541.182:541.183
O. Zaichenko, R. Stoika, N. Mitina, M. Lutsik, O. Shevchuk, V. Lobaz, N.Boiko
Lviv Polytechnic National University, Ukraine
Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv
Principal experimental approaches based on tailored synthesis of oligoperoxide surface-active substances and their application for obtaining polymeric and hybrid nanoscale carriers possessing targeted functionality and biocompatibility are presented. Molecular design of novel linear, block and comb-like oligoperoxide surfactants and derived coordinating complexes of transitional and rare earth metal cations is a convenient tool for synthesis of luminescent, magnetic and other functional nanocomposites with controlled size distribution, functionality, reactivity and biocompatibility. The methods developed provide combining the stage of formation of polymeric, metal and metal-oxide nanoparticles with the stage of their surface irreversible modification by functional surface-active oligoperoxides capable of binding physiologically active substances. The availability of reactive ditertiary peroxide fragments provides a possibility for functionalization of developed nanoparticles that were applied for studying phagocytosis, surface markers of pathological cells, and targeted delivery of drugs and antimicrobial remedies.
Key words: oligoperoxide surfactants, metal complexes, radical emulsion polymerization, homogeneous nucleation, functional colloidal particles, «core-shell» polymerization, phagocytosis, drug delivery.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
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ISSN 1995-5537
“Biotechnology” journal Vol. 1, No.1, 2008
P. 80-84, Bibliography 14, Ukrainian.
Universal Decimal classification: 578.245:577.1113.7:57.086.833
ESTIMATION OF EFFECTIVENESS OF EXPERIMENTAL DEVICE FOR OBTAINING OF TYPE I INTERFERONS
Yu. M. Penchuk, O. V. Karpov V. M. Povodzinsky S. V. Veriovka, Z. R. Ul’berg
National Universyti of Food Technologies, Kyiv
Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv
Kolomiychenko Institute of Otolaringology of Academy of Medical Sciences of Ukraine, Kyiv
Ovcharenko Institute of Biocolloid Chemisrty
Ovcharenko Institute of Biocolloid Chemisrty of the Nationale Academy of Sciences of Ukraine, Kyiv
The authors describe an experimental device with computer assisted action for cell cultivation and induced type I interferon (IFN) synthesis. The device is based on the use of previously elaborated particles bearing an immobilized complex inductor of repeated action.
The device is shown to be effective for cultivation of both monolayer and suspended cells producing the IFN. Physiological conditions of both cell types cultivation are investigated. The authors conclude their device to be useful for obtaining of biologically active substances by this technology.
Key words: interferon, inductor, immobilization, cpheron, cell culture, roller cultivation, mononuclears.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
- ABILITIES OF BIOTECHNOLOGICAL SYSTEM BASED ON Erwinia carotovora, ITS BACTERIOPHAGES AND PLASMIDS F. I. Tovkach
- ANALYSIS MYCOTOXINS: PREPARATION OF SAMPLES M. F. Starodub, L. M. Pilipenko, O. V. Egorova, I. V. Pilipenko, O. S. Gojster, G. O. Chmel’nitsky
- CONDUCTOMETRIC BIOSENSOR BASED ON THE THREENZYME SYSTEM FOR DETERMINATION OF SUCROSE O. O. Soldatkin, V. M. Peshkova, S. V. Dzyadevych, H. V. Elska
- CELL THERAPY OF HEART PATHOL L. L. Lukash