ENCAPSULATİON OF LAUROCERASUS OFFİCİNALİS AND İTS ANTİPROLİFERATİVE EFFECT ON COLORECTAL CANCER CELL LİNE Deniz ÖZDEMİR, Esma Nur BULUT, Seher SARUHAN, İkbal ABDURRAHMANOĞLU, Can Ali AĞCA*

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cover biotech acta general

Biotechnologia Acta Т. 19, No. 3, 2026
P. 46-55, Bibliography 38 , Engl.
UDC: 615.28:577.1:582.711.71
doi: https://doi.org/10.15407/biotech19.03.046

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ENCAPSULATİON OF LAUROCERASUS OFFİCİNALİS AND İTS ANTİPROLİFERATİVE EFFECT ON COLORECTAL CANCER CELL LİNE

Deniz ÖZDEMİR¹, Esma Nur BULUT ², Seher SARUHAN ¹, İkbal ABDURRAHMANOĞLU ³, Can Ali AĞCA^3*

¹Department of Molecular Biology and Genetics, Science Institute, Bingol University, Türkiye
²Department of Food Technology, Food Agriculture and Livestock Vocational School, Bingöl University, TÜRKİYE
³ Bingöl University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Türkiye

Aim. Laurocerasus officinalis, also known as cherry laurel, is frequently used in cancer treatment due to its high antioxidant capacity. Encapsulation technology aims to protect phenolic compounds from external factors, such as heat, light, and moisture, while preserving their bioactive properties. This study aimed to enhance the bioavailability of Laurocerasus officinalis fruit extract through starch-based encapsulation and to evaluate its cytotoxic, antiproliferative, and anti-migratory effects on the HCT-116 colorectal cancer cell line.

Methods. Laurocerasus officinalis was encapsulated and characterized by FT-IR and SEM analyses. Cytotoxicity of cherry laurel extract encapsulation in HCT-116 cells was assessed using CVDK-8, cell migration by wound healing assay, and cell death by acridine orange/ethidium bromide staining.

Results. The TPC value of cherry laurel fruit was determined as 13407.05 mg GAE/kg, while the TPC value of the starch-encapsulated extract was obtained as 1216.67 mg GAE/kg. On the other hand, encapsulation of cherry laurel fruit extract demonstrated an antiproliferative effect, reducing HCT-116 cell viability in a dose- and time-dependent manner. Further analyses supported the finding that encapsulated cherry laurel extract reduced cell migration and increased apoptotic cell death.

Conclusion. Overall, these findings suggest that the encapsulated Laurocerasus officinalis extract exhibits promising antiproliferative potential against HCT-116 cells; however, additional in vitro and in vivo studies are required to elucidate its mechanisms of action further and confirm its therapeutic efficacy.

Keywords: Cherry Laurel, Colorectal Cancer, Encapsulation, Cell Migration, Apoptosis.

Deniz ÖZDEMİR https://orcid.org/0000-0001-7659-742X
Esma Nur BULUT https://orcid.org/0000-0002-1740-7152
Seher SARUHAN https://orcid.org/0000-0003-1641-8519
İkbal ABDURRAHMANOĞLU https://orcid.org/0009-0006-3190-6173
Can Ali AĞCA ORCID https://orcid.org/0000-0002-0244-3767

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