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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta
V. 7, No 5, 2014

"Biotechnologia Acta" v. 7, no 5, 2014
doi: 10.15407/biotech7.05.094
Р. 94-100, Bibliography 21, English.
Universal Decimal classification: 579:662.7

COMPOSITION AND ACTIVITY OF BACTERIAL COMMUNITY OF COAL TAILING

Blayda I. A.

Mechnikov Odesa National University, Ukraine

The aim of this research was to study the composition of aboriginal bacterial community of coal tailing and to evaluate lixiviation activity of different groups of microorganisms belonging to this community. Using standard microbiological techniques we obtained and quantified the saving cultures of microorganisms from different physiological groups — filamentous fungi, heterotrophic microorganisms, mesophilic and thermophilic moderately acidophilic sulfur-oxidizing chemolithotrophic bacteria. Their oxidative activity was also established. The optimal results were achieved for collective leaching of rare and heavy metals into the solution under thermophilic conditions, which are favorable for the growth and activity of Sulfobacillus and under mesophilic conditions with the usage of ferrous iron as an energy substrate. This confirms the leading role of A. ferrooxidans in the processes of bacterial leaching of metals. Comparing our results with the available literature data we made a conclusion that the qualitative composition of acidophilic chemolithotrophic bacteria living in technogenic waste did not differ from the microbiocenose structure of natural sulfide ores.

Key words: coal tailing, the aboriginal bacterial community, chemolithotrophic acidophilic bacteria, leaching activity.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008